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Etude des anomalies chromosomiques chez Crassostrea gigas ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu'il existe, chez l'huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Anomalies; Aneuploïdie; Atrazine; Huîtres creuses; Crassostrea gigas.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00199/31013/29417.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas - Contrat de Plan Région Poitou-Charentes 2000-2006. Convention 2000 ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Bouilly, Karine; Sabatier, Sebastien; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00043/15474/12853.pdf
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Etude des anomalies chromosomiques chez Crassostrea gigas. Contrat Région Poitou-Charentes 2000-2006. Convention 99 ArchiMer
Lapegue, Sylvie; Mccombie, Helen; Leitao, Alexandra; Heurtebise, Serge; Boudry, Pierre; Thiriot Quievreux, Catherine; Gerard, Andre.
Divers travaux menés depuis 1984 montrent qu’il existe, chez l’huître creuse Crassostrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d’aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d’aneuploïdie. Cependant, de nombreux points concernant ce phénomène...
Tipo: Text Palavras-chave: Génétique; Chromosomes; Aneuploïdie; Huîtres creuses; Crassostrea gigas.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00043/15473/12852.pdf
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Etude des processus de dérive et de sélection liés aux pratiques d'élevage en écloserie d'huître creuse ArchiMer
Boudry, Pierre.
Genetic consequences of production of Pacific oyster larval in hatchery: drift and selective pressures related to rearing practices. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors were examined: the effects of discarding the smallest larvae (i.e. culling) and temperature effects. A mixed-family approach was used in order to infer the genetic composition of the larval population. The results show that high polymorphic microsatellite-based family assignment is a powerful tool for the study of bivalve larvae genetics. Culling by selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss if parentage assignment is not introduced as a...
Tipo: Text Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-1459.pdf
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Etude des ressources génétiques chez les huîtres creuses ArchiMer
Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Lam, Katherine; Mccombie, Helen; Leitao, Alexandra; Gerard, Andre.
L'étude de la variabilité génétique de l'huître creuse Crassostrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 'ro de la production mondiale d'huîtres. Introduite en France au début des années 1970 pour remplacer l'huître portugaise Crassostrea angulata, c'est aujourd'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n'a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la...
Tipo: Text Palavras-chave: Génétique; Huîtres creuses; Crassostrea gigas; Crassostrea gasar; Variabilité génétique.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00199/31008/29411.pdf
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Etude du niveau d'aneuploïdie dans les populations des zones de captage du bassin de Marennes-Oléron - Contrat Plan Etat Région Poitou-Charentes 1999 - Rapport 1999 ArchiMer
Lapegue, Sylvie; Thiriot Quievreux, Catherine; Mccombie, Helen; Heurtebise, Serge; Boudry, Pierre; Robert, Stephane; Soletchnik, Patrick; Goulletquer, Philippe; Gerard, Andre.
Divers travaux, menés depuis 1984, montrent qu'il existe, chez l'huître creuse Crassotrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Le but de l'expérimentation menée en 1999 est de faire un...
Tipo: Text Palavras-chave: Génétique; Aneuploïdie; Génétique des populations; Biochimie; Marennes-Oléron; Huître creuse; Crassostrea gigas.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00044/15522/12909.pdf
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Etude du polymorphisme des gènes de l'amylase chez Crassostrea gigas : relation avec les paramètres physiologiques de l'assimilation et croissance ArchiMer
Degremont, Lionel; Moal, Jeanne; Daniel, Jean-yves; Boudry, Pierre; Van Vormhoudt, A.; Sellos, D.; Samain, Jean-francois.
In the scope of the European program GENEPHYS, which aims to establish the relationship between the genetic base and the physiological characters in relation to the growth in Crassostrea gigas, it has been demonstrated that the most explanatory factors for the growth variability are assimilation performance and aneuploidy. A model linking the assimilation performances and the digestive equipment has been set up and more than 70% of the variability can be explained by two digestive enzymes: laminarinase and amylase. A number of laboratories have decided to focus on the latter.
Tipo: Text Palavras-chave: Croissance; Génétique; Polymorphisme; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3269.pdf
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Etude transcriptomique, génétique et fonctionnelle de la gamétogenèse chez l'huître creuse Crassostrea gigas ArchiMer
Bigot, L.; Dubos, M. P.; Dheilly, N.; Favrel, P.; Kellner, K.; Lelong, C.; Martinez, A. S.; Riviere, G.; Santerre, C.; Sourdaine, P.; Degremont, Lionel; Flahauw, Emilie; Hatt, Philippe-jacques; Heurtebise, Serge; Lapegue, Sylvie; Azzouzi, N.; Galibert, F.; Boudry, Pierre; Corporeau, Charlotte; Fabioux, Caroline; Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana.
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme du sexe chez une espèce hermaphrodite à protandrie irrégulière (2) la recherche de QTL de survie aux mortalités estivales ou à l'effort de reproduction dont le succès repose sur l'augmentation de la densité de marqueurs sur les cartes génétiques existantes (SNP en particulier) et sur les liens qui pourront être établis entre ces cartes de liaison et une carte physique (3) le développement d'outils pour valider la...
Tipo: Text Palavras-chave: Génétique; Huître creuse; Crassostrea gigas; Gamétogenèse; QTL; Marqueur; Physiologie.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21781/19355.pdf
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Etudes des bases génétiques et de la varibailité des caractères physiologiques impliqués dans la croissance chez l'huître creuse Crassostrea gigas ArchiMer
Collet, Bertrand; Boudry, Pierre; Bougrier, Serge; Heurtebise, Serge; Phelipot, Pascal; Ledu, Christophe; Morand, Berenice; Heral, Maurice; Gerard, Andre.
A European programme (GENEPHYS) was launched in 1996 to study the genetic basis and variability of physiological characters involved in growth in Crassostrea gigas. This research programme was a prerequisite for any genetic selection programme on physiological functions. Research groups from Ireland, England, Greece and France are involved in this project and participate in genetic analyses (allozymes and microsatellites) and physiological analyses (ecophysiology, enzymology, stress proteins). Ifremer at La Tremblade was responsible for producing the biological material while Ifremer-CREMA (human-influenced marine ecosystems research centre) was responsible for carrying out non-destructive physiological analyses.
Tipo: Text Palavras-chave: Sélection génétique; Croissance; Crassostrea gias; Génétique.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3243.pdf
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European flat oyster - Ostrea edulis ArchiMer
Lapegue, Sylvie; Beaumont, A.; Boudry, Pierre; Goulletquer, Philippe.
The European flat oyster, Ostrea edulis, a native of Europe, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and in the whole Mediterranean Basin; It has been a harvested species for at least 6000 years. Natural populations are also observed in eastern North America, from Maine to Rhode Island, following intentional introductions in the 1940s and 1950s. The species was also introduced in Canada for aquaculture purpose 30 years ago and some populations naturalised in Nova Scotia, New Brunswick and British Columbia. These stocks were imported from naturalised populations in Maine whose ancestors originated in the Netherlands.
Tipo: Text Palavras-chave: Genetics; Ecology; Biology; Ostrea edulis; Oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3321.pdf
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Evidence for natural hybrtidisation between the two oyster sub-species Crassostrea gigas and Crassostrea angulata in Southern Europe ArchiMer
Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie.
Crassostrea angulata and Crassostrea gigas, two cupped oyster taxa of Asian origin (O'Foighil et al, 1998), were successively introduced into Europe during the XVIth and the XXth century respectively (Héral and Grizel, 1991). To date, populations presumed to be C. angulata remain only in the south of Spain and Portugal, while the geographic range of C. gigas is expending, notably due to aquaculture. The two taxa can be distinguished by RFLP analysis of the mitochondrial Cytochrome Oxydase I locus (COI). This marker revealed strong genetic differentiation between Asian populations of the two taxa (Boudry et al., 1998). In contrast, nuclear markers showed a much lower genetic differentiation (Huvet et al, in press). The absence of any reproductive barrier...
Tipo: Text Palavras-chave: Microsatellite; Genetic; Crassostrea angulata; Crassostrea gigas; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3453.pdf
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Evidence for the presence of the Portuguese oyster, Crassostrea angulata, in northern China ArchiMer
Lapegue, Sylvie; Batista, Frederico; Heurtebise, Serge; Yu, Ziniu; Boudry, Pierre.
The Pacific oyster, Crassostrea gigas (Thunberg), and the Portuguese oyster, C. angulata (Lamarck), are two closely related taxa. Although these two taxa were both introduced from Asia into Europe. one (C. gigas) was voluntarily introduced in the early 1970s, whereas the other (C. angulata) was presumed to be present in Europe for at least four centuries, but nearly disappeared because of disease. Few C. angulata populations remained in southern Portugal, Spain and Morocco and their putative origin was traced in Taiwan. The present paper reports evidence for its presence in Northern China. We reanalyzed recently published mitochondrial cytochrome oxidase C subunit I (COI) sequence data from presumed Dalianwan oysters (C. talienwhanensis) and compared them...
Tipo: Text Palavras-chave: Phylogeography; Cytochrome oxidase C subunit 1 gene; Crassostrea talienwhanensis; Gigas; Crosstrea; Crassostrea angulata; Cupped oysters.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-3173.pdf
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Evidence of differential chromosome loss in aneuploid karyotypes of the Pacific oyster, Crassostrea gigas ArchiMer
Leitao, Alexandra; Boudry, Pierre; Thiriot Quievreux, Catherine.
The G-banding technique was performed on aneuploid karyotypes from gill tissue of the Pacific oyster, Crassostrea gigas, to assess whether chromosome losses could be explained by differential chromosomal susceptibility and to clarify the negative correlation between aneuploidy and growth rate previously reported in different populations of this oyster. The study of 95 G-banded aneuploid karyotypes showed that only 4 of the 10 chromosome pairs (viz. 1, 5, 9, and 10) of C. gigas were affected by the loss of one homologous chromosome. Pairs 1, 9, and 10, which were lost in 56, 33, and 44% of cases, respectively, may be considered to be differentially affected. Hypotheses on this differential chromosomal susceptibility are discussed.
Tipo: Text Palavras-chave: Oyster; Karyotype; G banding; Growth; Aneuploidy; Huître; Caryotype; Bandes G; Croissance; Aneuploïdie.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-742.pdf
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Evidence of response to unintentional selection for faster development and inbreeding depression in Crassostrea gigas larvae ArchiMer
Taris, Nicolas; Batista, F; Boudry, Pierre.
Underlying consequences of domestication and artificial selection still remain largely unexplored in most aquacultured species. For species with a two phase life cycle, including the Pacific oyster Crassostrea gigas, most genetic studies have focused on the post-metamorphosis juvenile and adult stages, but relatively few considered the larval stage. To assess the consequence of hatchery practices on larval characters, especially growth, we performed a phenotypic study on larval progenies derived from crosses between Pacific oysters from natural beds and farmed Pacific oysters selected for desirable production traits such as rapid growth, for over seven generations. A set of three microsatellite loci was used to compare the genetic variability between the...
Tipo: Text Palavras-chave: Crassostrea gigas; Inbreeding depression; Selection; Domestication; Larval stage.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3848.pdf
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Experimental evidence for a genetic basis to differences in aneuploidy in the Pacific oyster (Crassostrea gigas) ArchiMer
Leitao, Alexandra; Boudry, Pierre; Mccombie, Helen; Gerard, Andre; Thiriot-quievreux, Catherine.
Aneuploidy has been previously reported in wild and cultivated Pacific oysters, Crassostrea gigas, and has been shown to be negatively correlated with growth. This is especially important since high variability of growth rate is one of the major problems in the aquacultural production of this species. The existence of a genetic basis for the observed differences in aneuploidy was first investigated through the comparative study of six full-sib families, with mean individual weights ranging from 0.59 to 1.49 g. The slowest growing family was found to have the highest level of aneuploidy. Significant differences in aneuploidy were also found among families when individuals with the same growth rate were sampled. This supports the hypothesis of the existence...
Tipo: Text Palavras-chave: Oyster; Growth; Genetics; Full sib families; Crassostrea gigas; Aneuploidy; Huître; Croissance; Génétique; Famille de plein frères; Crassostrea gigas; Aneuploïdie.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-2700.pdf
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Exploitation par la pêche de coquillages d’écloserie en rade de Brest : quels acteurs pour quelles perspectives ? ArchiMer
Danto, Anatole; Boudry, Pierre; Ragueneau, Olivier; Maze, Camille.
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00375/48577/48899.pdf
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Exploring local reproductive differentiation of Pacific oyster population recently settled in northern Europe by transcriptomic approach ArchiMer
Sussarellu, Rossana; Huvet, Arnaud; Lelong, Christophe; Pernet, Fabrice; Quillen, Virgile; Lapegue, Sylvie; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre.
Originating from north-eastern Asia, the Pacific oyster Crassostrea gigas has been introduced, mainly for aquaculture purpose, into a large number of countries. Although highly variable, its invasive nature has been reported in an increasing number of coastal areas, notably. Northern Europe. We set up a common garden experiment based on the comparison of progenies of Pacific oysters sampled in France, where populations settled since the introduction of the species in the 70’s, and Denmark, where established populations were observed since the beginning of 90s. Pure and hybrid progenies, resulting from crosses within and between adults sampled in both populations, were studied for their sex-ratio, condition index, transcriptomics, and biochemical parameters
Tipo: Text
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27815/26008.pdf
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Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters ArchiMer
Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf
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Factors influencing disease-induced mortality of Pacific oysters Crassostrea gigas ArchiMer
Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice.
Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented...
Tipo: Text Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf
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FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax) ArchiMer
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Tipo: Text
Ano: 2010
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